ESR2 Beta 3 Polyclonal antibody

ESR2 Beta 3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 55441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman cervix tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55441-1-AP targets ESR2 Beta 3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2
计算分子量 58 kDa
GenBank蛋白编号NM_001437
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_2881334
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92731
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual

实验方案

Product Specific Protocols
IHC protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
IF protocol for ESR2 Beta 3 antibody 55441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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