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验证数据展示

  • FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ESR2 antibody (83447-3-RR, Clone: 240412F2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

    IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

    IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83447-3-PBS

    Affinity and Kinetic Characterization of 83447-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83447-3-PBS against Human ESR2 was performed. The affinity constant is 16.6 pM.

  • Affinity and Kinetic Characterization of 83447-3-RR

    Affinity and Kinetic Characterization of 83447-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83447-3-RR against Human ESR2 were performed. The affinity constant is 0.22 nM.

ESR2 Recombinant antibody, PBS Only

ESR2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83447-3-PBS

产品说明书

CloneNo. 240412F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

240412F2, ER BETA, Erb, ER-beta, ERβ

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83447-3-PBS targets ESR2 in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ESR2 fusion protein Ag5103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 240412F2, ER BETA, Erb, ER-beta, ERβ
计算分子量 59 kDa
GenBank蛋白编号BC024181
基因名称 ESR2
Gene ID (NCBI) 2100
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92731
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.