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ESR2 Polyclonal antibody

ESR2 Polyclonal Antibody for WB, ELISA
Cat No. 14007-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, ELISA and More (3)

ER BETA, Erb, ER-beta, ERβ, ESTRB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inMCF-7 cells, SKOV-3 cells, mouse testis tissue, mouse brain tissue, PC-3 cells, SH-SY5Y cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14007-1-AP targets ESR2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IF, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, sheep
免疫原

CatNo: Ag5103

Product name: Recombinant human ESR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC024181

Sequence: MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGMRGNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 ER BETA, Erb, ER-beta, ERβ, ESTRB
计算分子量 59 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC024181
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_2102386
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92731
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoforms and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

实验方案

Product Specific Protocols
WB protocol for ESR2 antibody 14007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biomed Sci

Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.

Authors - Chunfang Zhang
humanIF

ACS Sens

Estrogen Receptor β-Targeted Near-Infrared Inherently Fluorescent Probe: A Potent Tool for Estrogen Receptor β Research.

Authors - Qiuyu Meng
humanWB,IHC

Int J Cancer

ERβ promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway

Authors - Yangwei Wang
  • KD Validated
mouseIHC

Int J Cancer

Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.

Authors - Tang Hexiao H
humanWB

Cell Death Dis

ERβ promotes Aβ degradation via the modulation of autophagy.

Authors - Yong Wei
humanWB,IHC

Fertil Steril

Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.

Authors - Miao Sun
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