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验证数据展示

  • WB analysis of K-562 using 15244-1-AP

    WB analysis of K-562 using 15244-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of HAP1 using 15244-1-AP

    WB analysis of HAP1 using 15244-1-AP

    WB result of ESD antibody (15244-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and ESD knockout HAP1 cells.

  • Featured Product
  • KD/KO验证

ESD Polyclonal antibody

ESD Polyclonal Antibody for WB, ELISA
Cat No. 15244-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HAP1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15244-1-AP targets ESD in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7457

Product name: Recombinant human ESD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-282 aa of BC001169

Sequence: MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQSASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDPQRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKSYPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYLNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 esterase D/formylglutathione hydrolase
别名 S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12
计算分子量 31 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC001169
基因名称 ESD
Gene ID (NCBI) 2098
RRIDAB_2101686
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

实验方案

Product Specific Protocols
WB protocol for ESD antibody 15244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols