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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

    WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included

ERp72 Monoclonal antibody, PBS Only

ERp72 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66365-1-PBS

产品说明书

CloneNo. 1D5F3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.

产品信息

66365-1-PBS targets ERp72 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6873

Product name: Recombinant human ERp72 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 294-645 aa of BC000425

Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 4
别名 Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4
计算分子量 73 kDa
观测分子量 72 kDa
GenBank蛋白编号BC000425
基因名称 ERp72
Gene ID (NCBI) 9601
RRIDAB_2881745
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13667
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.

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