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验证数据展示

  • WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

    WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

    IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included
  • IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

    IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDIA4/ERP72 antibody included
  • IF Staining of A431 using 14712-1-AP (same clone as 14712-1-PBS)

    IF Staining of A431 using 14712-1-AP (same clone as 14712-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

    IP experiment of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

    IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)

    FC experiment of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ERp72 Polyclonal antibody, PBS Only

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 14712-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PDIA4/ERP72 Ready-To-Use IHC Kit. PDIA4/ERP72 primary antibody included.

产品信息

14712-1-PBS targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6431

Product name: Recombinant human ERp72 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 294-645 aa of BC000425

Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein disulfide isomerase family A, member 4
别名 Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72
计算分子量 73 kDa
观测分子量 72 kDa
GenBank蛋白编号BC000425
基因名称 ERp72
Gene ID (NCBI) 9601
RRIDAB_2160973
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13667
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

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