验证数据展示
经过测试的应用
| Positive WB detected in | JAR cells, A549 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67289-1-Ig targets ERN2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag29452 Product name: Recombinant human ERN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 452-563 aa of BC157113 Sequence: SLSREKLWDSELHPEEKTPDSYLGLGPQDLLAASLTAVLLGGWILFVMRQQQPQVVEKQQETPLAPADFAHISQDAQSLHSGASRRSQKRLQSPSKQAQPLDDPEAEQLTVV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | endoplasmic reticulum to nucleus signaling 2 |
| 别名 | ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2 |
| 观测分子量 | 102 kDa |
| GenBank蛋白编号 | BC157113 |
| 基因名称 | ERN2 |
| Gene ID (NCBI) | 10595 |
| RRID | AB_2882555 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q76MJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ERN2 antibody 67289-1-Ig | Download protocol |
| WB protocol for ERN2 antibody 67289-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
