验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, HL-60 cells, HepG2 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85377-1-RR targets ERLIN1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11393 Product name: Recombinant human ERLIN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-346 aa of BC031791 Sequence: GHLAVYYRGGALLTSPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAVFDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNLMAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKKAVIEAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ER lipid raft associated 1 |
| 别名 | 242778B10, C10orf69, Endoplasmic reticulum lipid raft-associated protein 1, ER lipid raft associated 1, Erlin 1 |
| 计算分子量 | 346 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC031791 |
| 基因名称 | ERLIN1 |
| Gene ID (NCBI) | 10613 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75477 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERLIN1, also known as Erlin-1 or SPFH1, stands for "ER lipid raft-associated protein 1." It is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). The ER is a crucial organelle involved in protein synthesis, lipid metabolism, and calcium homeostasis. ERLIN1 is part of the SPFH (stomatin, prohibitin, flotillin, and HflK/C) domain-containing family of proteins, which are often associated with lipid rafts-microdomains within cellular membranes that are enriched in cholesterol and sphingolipids. ERLIN1 is involved in the regulation of protein degradation, particularly through the ER-associated degradation (ERAD) pathway (PMID: 37683630).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERLIN1 antibody 85377-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
