ERLIN1 Polyclonal antibody
ERLIN1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, COLO 320 cells, human kidney tissue, human ovary cancer cells, rat pancreas tissue, human brain tissue, mouse liver tissue, U-251 cells, U-397 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
17311-1-AP targets ERLIN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ERLIN1 fusion protein Ag11279 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ER lipid raft associated 1 |
| Synonyms | C10orf69, ER lipid raft associated 1, Erlin 1, ERLIN1, KE04, KEO4, Protein KE04, SPFH1 |
| Calculated Molecular Weight | 346 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC031791 |
| Gene Symbol | ERLIN1 |
| Gene ID (NCBI) | 10613 |
| RRID | AB_2098590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERLIN1 antibody 17311-1-AP | Download protocol |
| IHC protocol for ERLIN1 antibody 17311-1-AP | Download protocol |
| IF protocol for ERLIN1 antibody 17311-1-AP | Download protocol |
| IP protocol for ERLIN1 antibody 17311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation. | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex | ||
Mol Cell Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex. | ||
Elife RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells. | ||
PLoS Genet Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease. | ||
Front Mol Biosci Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients |
