Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody
Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (4)
Applications
IP, WB, ELISA and More (2)
Conjugate
Unconjugated
268
验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells |
| Positive IP detected in | Calyculin A treated PC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, sheep, pig |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase 3 |
| Synonyms | ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3 |
| Calculated Molecular Weight | 38-43 kDa |
| Observed Molecular Weight | 38-43 kDa |
| GenBank Accession Number | NM_002746 |
| Gene Symbol | MAPK3 |
| Gene ID (NCBI) | 5595 |
| RRID | AB_2881202 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27361 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Download protocol |
| IP protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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