验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells, U-251 cells, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10548-1-AP targets ERI3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0826 Product name: Recombinant human ERI3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC001072 Sequence: MVDGQPSLQQVLERVDEWMAKEGLLDPNVKSIFVTCGDWDLKVMLPGQCQYLGLPVADYFKQWINLKKAYSFAMGCWPKNGLLDMNKGLSLQHIGRPHSGIDDCKNIANIMKTLAYRGFIFKQTSKPF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exoribonuclease 3 |
| 别名 | EC:3.1.-.-, ERI1 exoribonuclease 3, exoribonuclease 3, PINT1, Prion interactor 1 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC001072 |
| 基因名称 | ERI3 |
| Gene ID (NCBI) | 79033 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43414 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERI3, officially designated as ERI1 exoribonuclease family member 3, is also known by several aliases, including enhanced RNAi three prime mRNA exonuclease homolog 3, exoribonuclease 3, PINT1, and PRNPIP (prion protein-interacting protein). As a member of the metazoan 3'hExo/ERI-1 family of 3' to 5' exonucleases, ERI3 enables RNA binding and exonuclease activity. It is predicted to be involved in the exonucleolytic trimming that generates the mature 3'-end of 5.8S rRNA from the tricistronic rRNA transcript. ERI3 overexpression promotes HCC cell proliferation, migration, and invasion and is strongly linked to a poor prognosis of the patients (PMID: 41540704).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERI3 antibody 10548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
