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ERI1 Polyclonal antibody

ERI1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14592-1-AP

Print datasheet

Synonyms

3 5 exonuclease ERI1, 3 5 exoribonuclease 1, 3EXO, 3'HEXO, Eri 1 homolog, ERI1, exoribonuclease 1, HEXO, Histone mRNA 3 exonuclease 1, Protein 3hExo, THEX1

验证数据展示

  • WB analysis of K-562 using 14592-1-AP

    WB analysis of K-562 using 14592-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14592-1-AP

    IHC staining of human liver cancer using 14592-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 14592-1-AP

    IF Staining of HepG2 using 14592-1-AP

    Immunofluorescent analysis of HepG2 cells using 14592-1-AP (ERI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells
Positive IHC detected inhuman liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14592-1-AP targets ERI1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen ERI1 fusion protein Ag6125 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exoribonuclease 1
Synonyms 3 5 exonuclease ERI1, 3 5 exoribonuclease 1, 3EXO, 3'HEXO, Eri 1 homolog, ERI1, exoribonuclease 1, HEXO, Histone mRNA 3 exonuclease 1, Protein 3hExo, THEX1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC035279
Gene Symbol ERI1
Gene ID (NCBI) 90459
RRIDAB_11182393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

实验方案

Product Specific Protocols
WB protocol for ERI1 antibody 14592-1-APDownload protocol
IHC protocol for ERI1 antibody 14592-1-APDownload protocol
IF protocol for ERI1 antibody 14592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols