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ERH Polyclonal antibody

ERH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15974-1-AP

Print datasheet

Synonyms

DROER, ERH

验证数据展示

  • WB analysis using 15974-1-AP

    WB analysis using 15974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15974-1-AP (ERH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15974-1-AP

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15974-1-AP

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

15974-1-AP targets ERH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Immunogen ERH fusion protein Ag8761 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enhancer of rudimentary homolog (Drosophila)
Synonyms DROER, ERH
Calculated Molecular Weight 104 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC014301
Gene Symbol ERH
Gene ID (NCBI) 2079
RRIDAB_2098556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERH antibody 15974-1-APDownload protocol
IHC protocol for ERH antibody 15974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Cell Sci

Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.

Authors - Valentina Iadevaia
View Article