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验证数据展示

  • WB analysis using 15974-1-AP

    WB analysis using 15974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15974-1-AP (ERH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15974-1-AP

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ERH antibody included
  • IHC staining of mouse testis using 15974-1-AP

    IHC staining of mouse testis using 15974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15974-1-AP (ERH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ERH antibody included

ERH Polyclonal antibody

ERH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15974-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

DROER, ERH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ERH Ready-To-Use IHC Kit. ERH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

15974-1-AP targets ERH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8761

Product name: Recombinant human ERH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC014301

Sequence: MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enhancer of rudimentary homolog (Drosophila)
别名 DROER, ERH
计算分子量 104 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC014301
基因名称 ERH
Gene ID (NCBI) 2079
RRIDAB_2098556
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP84090
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERH antibody 15974-1-APDownload protocol
IHC protocol for ERH antibody 15974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Cell Sci

Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.

Authors - Valentina Iadevaia
View Article