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ERGIC3 Polyclonal antibody

ERGIC3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16029-1-AP

Print datasheet

Synonyms

C20orf47, CGI 54, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

验证数据展示

  • WB analysis using 16029-1-AP

    WB analysis using 16029-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16029-1-AP (ERGIC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16029-1-AP

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16029-1-AP

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 16029-1-AP

    IF Staining of HepG2 using 16029-1-AP

    Immunofluorescent analysis of HepG2 cells using 16029-1-AP (ERGIC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16029-1-AP targets ERGIC3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ERGIC3 fusion protein Ag8893 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ERGIC and golgi 3
Synonyms C20orf47, CGI 54, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009765
Gene Symbol ERGIC3
Gene ID (NCBI) 51614
RRIDAB_2098446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y282
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer (PMID: 23374247). The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro. ERGIC3 may play an active role in the development and progression of lung cancer (PMID: 23374247). The MW of this protein is 50 kDa, and this antibody specially recognises the 50 kDa protein.

实验方案

Product Specific Protocols
WB protocol for ERGIC3 antibody 16029-1-APDownload protocol
IHC protocol for ERGIC3 antibody 16029-1-APDownload protocol
IF protocol for ERGIC3 antibody 16029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of TRAMs as Sphingolipid-Binding Proteins Using a Photoactivatable and Clickable Short Chain Ceramide Analog.

Authors - Yaqin Deng
View Article