Various lysates were subjected to SDS PAGE followed by western blot with 86688-1-RR (ERCC6L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86688-1-PBS in a different storage buffer formulation.

The mean ERCC6L concentration was determined to be 355.50 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 346.30 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 214.50 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 409.90 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load and 318.20 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

Sandwich ELISA standard curve of MP02582-2, Human ERCC6L Recombinant Matched Antibody Pair - PBS only. 86688-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8223. 86688-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

IP result of anti-ERCC6L (IP:86688-1-RR, 4ug; Detection:86688-1-RR 1:2000) with K-562 cells lysate 1640 ug. This data was developed using the same antibody clone with 86688-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 86688-1-RR against Human ERCC6L were performed. The affinity constant is 0.79 nM.