验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24291-1-AP targets ERCC6/CSB in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18009 Product name: Recombinant human ERCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1144-1493 aa of BC127104 Sequence: DESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | excision repair cross-complementing rodent repair deficiency, complementation group 6 |
| 别名 | ERCC6, ARMD5, ATP dependent helicase ERCC6, Chimeric CSB-PGBD3 protein, Chimeric ERCC6-PGBD3 protein |
| 计算分子量 | 1493 aa, 168 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC127104 |
| 基因名称 | ERCC6/CSB |
| Gene ID (NCBI) | 2074 |
| RRID | AB_2879476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q03468 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| WB protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons | ||
Int J Mol Sci A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches. | ||
Front Mol Biosci Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.
| ||
Front Oncol Studies on the functionality of the TC-NER ERCC6-M1097V protein variant frequently found in Louisiana patients with PCa upon UV damage.
| ||
Life Sci Alliance Elevated glucose increases genomic instability by inhibiting nucleotide excision repair. |
