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验证数据展示

  • WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

    WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 10580-1-AP (same clone as 10580-1-PBS)

    IP experiment of A431 using 10580-1-AP (same clone as 10580-1-PBS)

    IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 10580-1-AP (same clone as 10580-1-PBS)

    IHC staining of human stomach using 10580-1-AP (same clone as 10580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10580-1-AP (ERCC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ERCC3 Polyclonal antibody, PBS Only

ERCC3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10580-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

BTF2, BTF2 p89, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10580-1-PBS targets ERCC3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0876

Product name: Recombinant human ERCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 483-782 aa of BC008820

Sequence: LIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
别名 BTF2, BTF2 p89, DNA 3'-5' helicase/translocase XPB, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells
计算分子量 89 kDa
观测分子量 89 kDa
GenBank蛋白编号BC008820
基因名称 ERCC3
Gene ID (NCBI) 2071
RRIDAB_2262174
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19447
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].

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