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验证数据展示

  • WB analysis using 87175-3-RR (same clone as 87175-3-PBS)

    WB analysis using 87175-3-RR (same clone as 87175-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87175-3-RR (ERCC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87175-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87175-3-RR (same clone as 87175-3-PBS)

    IF Staining of HeLa using 87175-3-RR (same clone as 87175-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERCC1 antibody (87175-3-RR, Clone: 252372B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87175-3-PBS in a different storage buffer formulation.

  • Affinity of 87175-3-RR

    Affinity of 87175-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87175-3-RR against Human ERCC1 were performed. The affinity constant is below 1 pM.

ERCC1 Recombinant monoclonal antibody, PBS Only

ERCC1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87175-3-PBS

产品说明书

CloneNo. 252372B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

COFS4, DNA excision repair protein ERCC-1, RAD10, UV20

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87175-3-PBS targets ERCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6112

Product name: Recombinant human ERCC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC052813

Sequence: MDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKVRALGKNPRSWGKERAPNKHNLRPQSFKVKKEPKTRHSGFRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
别名 COFS4, DNA excision repair protein ERCC-1, RAD10, UV20
计算分子量 33 kDa
观测分子量40 kDa
GenBank蛋白编号BC052813
基因名称 ERCC1
Gene ID (NCBI) 2067
RRIDAB_3745390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07992
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005). The calculated molecular weight of ERCC1 is 33 kDa, but the modified ERCC1 is about38 kDa.

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