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ERC2 Polyclonal antibody

ERC2 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21396-1-AP

Print datasheet

Synonyms

CAST, CAST1, ELKSL, ERC protein 2, ERC2, KIAA0378, SPBC110, Spc110

验证数据展示

  • WB analysis of human brain using 21396-1-AP

    WB analysis of human brain using 21396-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21396-1-AP

    IF Staining of HeLa using 21396-1-AP

    Immunofluorescent analysis of Hela cells, using ERC2 antibody 21396-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21396-1-AP is ERC2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen ERC2 fusion protein Ag16080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ELKS/RAB6-interacting/CAST family member 2
Synonyms CAST, CAST1, ELKSL, ERC protein 2, ERC2, KIAA0378, SPBC110, Spc110
Calculated Molecular Weight 957 aa, 111 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC111550
Gene Symbol ERC2
Gene ID (NCBI) 26059
RRIDAB_10859782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15083
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERC2 antibody 21396-1-APDownload protocol
IF protocol for ERC2 antibody 21396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols