验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, U2OS cells, MCF-7 cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87639-1-RR targets ERBB2IP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5491 Product name: Recombinant human ERBIN protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 950-1100 aa of NM_001253697.1 Sequence: HNPEEPNIIRGPTSGPQSAPQIYGPPQYNIQYSSSAAVKDTLWHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQRQSSVSSTASVNLGDPGSTRRAQIP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | erbb2 interacting protein |
| 别名 | LAP2, Densin 180 like protein, Densin-180-like protein, Erbb2-interacting protein, ERBIN |
| 计算分子量 | 158 kDa |
| 观测分子量 | 150-180 kDa |
| GenBank蛋白编号 | NM_001253697.1 |
| 基因名称 | ERBB2IP |
| Gene ID (NCBI) | 55914 |
| RRID | AB_3745668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96RT1-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERBB2IP antibody 87639-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
