验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13821-1-AP targets ARTS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4918 Product name: Recombinant human ERAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 642-941 aa of BC030775 Sequence: NAFQLVSIGKLSIEKALDLSLYLKHETEIMPVFQGLNELIPMYKLMEKRDMNEVETQFKAFLIRLLRDLIDKQTWTDEGSVSERMLRSQLLLLACVHNYQPCVQRAEGYFRKWKESNGNLSLPVDVTLAVFAVGAQSTEGWDFLYSKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILTLIGRNPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQTIETIEENIGWMDKNFDKIRVWLQSEKLERM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endoplasmic reticulum aminopeptidase 1 |
| 别名 | A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase |
| 计算分子量 | 107 kDa |
| 观测分子量 | 106 kDa |
| GenBank蛋白编号 | BC030775 |
| 基因名称 | ARTS1/ERAP1 |
| Gene ID (NCBI) | 51752 |
| RRID | AB_2293531 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZ08 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| IP protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| WB protocol for ARTS1 antibody 13821-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autoimmunity Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens. |
