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ARTS1 Polyclonal antibody

ARTS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13821-1-AP

Print datasheet

Synonyms

A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase

验证数据展示

  • WB analysis of K-562 using 13821-1-AP

    WB analysis of K-562 using 13821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13821-1-AP (ARTS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 13821-1-AP

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary tumor using 13821-1-AP

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of Jurkat using 13821-1-AP

    IP experiment of Jurkat using 13821-1-AP

    IP result of anti-ARTS1 (IP:13821-1-AP, 4ug; Detection:13821-1-AP 1:600) with Jurkat cells lysate 3600ug.

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13821-1-AP targets ARTS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARTS1 fusion protein Ag4918 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoplasmic reticulum aminopeptidase 1
Synonyms A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC030775
Gene Symbol ERAP1
Gene ID (NCBI) 51752
RRIDAB_2293531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).

实验方案

Product Specific Protocols
WB protocol for ARTS1 antibody 13821-1-APDownload protocol
IHC protocol for ARTS1 antibody 13821-1-APDownload protocol
IP protocol for ARTS1 antibody 13821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

ERAP1-ERAP2 Dimerization Increases Peptide-Trimming Efficiency.

Authors - Irini Evnouchidou
View Article

Autoimmunity

Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.

Authors - Seregin Sergey S SS
View Article