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ERAL1 Polyclonal antibody

ERAL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29465-1-AP

Print datasheet

Synonyms

CEGA, Conserved ERA like GTPase, ERA, Era G protein like 1 (E. coli), ERA W, ERAL1, ERAL1A, hERA, HERA A, HERA B

验证数据展示

  • WB analysis of HEK-293T using 29465-1-AP

    WB analysis of HEK-293T using 29465-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29465-1-AP

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 29465-1-AP

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29465-1-AP targets ERAL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen ERAL1 fusion protein Ag30720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Era G-protein-like 1 (E. coli)
Synonyms CEGA, Conserved ERA like GTPase, ERA, Era G protein like 1 (E. coli), ERA W, ERAL1, ERAL1A, hERA, HERA A, HERA B
Calculated Molecular Weight 437 aa, 48 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC019094
Gene Symbol ERAL1
Gene ID (NCBI) 26284
RRIDAB_2918311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. ERAL1 localizes to the mitochondrionmitoribosomal subunit, and binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of ERAL1 has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis.

实验方案

Product Specific Protocols
WB protocol for ERAL1 antibody 29465-1-APDownload protocol
IHC protocol for ERAL1 antibody 29465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols