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EPX Polyclonal antibody

EPX Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, pig, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29755-1-AP

Print datasheet

Synonyms

eosinophil peroxidase, EPER, EPO, EPP, EPX, EPX PEN

验证数据展示

  • WB analysis using 29755-1-AP

    WB analysis using 29755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29755-1-AP (EPX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29755-1-AP targets EPX in WB, IHC, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, pig, mouse
Immunogen EPX fusion protein Ag30561 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eosinophil peroxidase
Synonyms eosinophil peroxidase, EPER, EPO, EPP, EPX, EPX PEN
Calculated Molecular Weight 81 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberNM_000502
Gene Symbol EPX
Gene ID (NCBI) 8288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPX antibody 29755-1-APDownload protocol
IHC protocol for EPX antibody 29755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols