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验证数据展示

  • WB analysis using 29755-1-AP

    WB analysis using 29755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29755-1-AP (EPX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using 29755-1-AP

    IF Staining of mouse lung using 29755-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using EPX antibody (29755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPX Polyclonal antibody

EPX Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29755-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, IF-P, ELISA

别名

EPX/ PERE, eosinophil peroxidase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29755-1-AP targets EPX in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag30561

Product name: Recombinant human EPX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 580-673 aa of NM_000502

Sequence: LSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLLPGARVGPLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eosinophil peroxidase
别名 EPX/ PERE, eosinophil peroxidase
计算分子量 81 kDa
观测分子量55 kDa
GenBank蛋白编号NM_000502
基因名称 EPX
Gene ID (NCBI) 8288
RRIDAB_3086154
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EPX antibody 29755-1-APDownload protocol
WB protocol for EPX antibody 29755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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