验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HT-29 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33149-1-AP targets EPS8L1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38144 Product name: Recombinant human EPS8L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-596 aa of BC015763 Sequence: LTSDAVALLRDNVTPRENELWTSLGDSWTRPGLELSPEEGPPYRPEFFSGWEPPVTDPQSRAWEDPVEKQLQHERRRRQQSAPQVAVNGHRDLEPESEPQLESETAGKWVLCNYDFQARNSSELSVKQRDVLEVLDDSRKWWKVRDPAGQEGYVPYNILTPYPGPRLHHSQSPARSLNSTPPPPPAPAPAPPPALARPRWDRPRWDSCDSLNGLDPSEKEKFSQMLIVNEELQARLAQGRSGPSRAVPGPRAPEPQLSPGSDASEVRAWLQAKGFSSGTVDALGVLTGAQLFSLQKEELRAVSPEEGARVYSQVTVQRSLLEDKEKVSELEAVMEKQKKKVEGEVEMEVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EPS8-like 1 |
| 别名 | DRC3, Epidermal growth factor receptor kinase substrate 8-like protein 1, Epidermal growth factor receptor pathway substrate 8-related protein 1, EPS8-like protein 1, EPS8R1 |
| 计算分子量 | 80 kDa |
| 观测分子量 | 80-85 kDa |
| GenBank蛋白编号 | BC015763 |
| 基因名称 | EPS8L1 |
| Gene ID (NCBI) | 54869 |
| RRID | AB_3742872 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8TE68 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPS8L1 encodes Epidermal Growth Factor Receptor Kinase Substrate 8-like Protein 1, a protein related to EPS8 and positioned within the EPS8 signaling adaptor family. Its biology is centered on signaling at the cell cortex, where it is connected to ruffle membrane and cytosolic compartments and is associated with positive regulation of ruffle assembly. The protein contains multiple interaction-related domains, including a PTB/PI domain, phosphotyrosine-binding domain, SH3 domain, and SAM domain, supporting a role in signal-dependent molecular interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPS8L1 antibody 33149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
