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验证数据展示

  • WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

    WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

    50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66975-1-Ig (same clone as 66975-1-PBS)

    IF Staining of HeLa using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPO antibody (66975-1-Ig, Clone: 1A11F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

EPO/Erythropoietin Monoclonal antibody, PBS Only

EPO/Erythropoietin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66975-1-PBS

产品说明书

CloneNo. 1A11F3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EPO, MVCD2, erythropoietin, Epoetin, EP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66975-1-PBS targets EPO/Erythropoietin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23054

Product name: Recombinant human EPO protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 29-193 aa of BC093628

Sequence: PPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 erythropoietin
别名 EPO, MVCD2, erythropoietin, Epoetin, EP
计算分子量 193 aa, 21 kDa
GenBank蛋白编号BC093628
基因名称 EPO/Erythropoietin
Gene ID (NCBI) 2056
RRIDAB_2882295
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01588
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.