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验证数据展示

  • WB analysis using 31334-1-AP (same clone as 31334-1-PBS)

    WB analysis using 31334-1-AP (same clone as 31334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31334-1-AP (EPN2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    IHC staining of mouse lung using 31334-1-AP (same clone as 31334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 31334-1-AP (same clone as 31334-1-PBS)

    IF Staining of HepG2 using 31334-1-AP (same clone as 31334-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPN2 antibody (31334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31334-1-PBS in a different storage buffer formulation.

EPN2 Polyclonal antibody, PBS Only

EPN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31334-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EHB21, EPS 15 interacting protein 2, EPS-15-interacting protein 2, epsin 2, Epsin-2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31334-1-PBS targets EPN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35194

Product name: Recombinant human EPN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 252-448 aa of BC093972

Sequence: LEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATAQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epsin 2
别名 EHB21, EPS 15 interacting protein 2, EPS-15-interacting protein 2, epsin 2, Epsin-2
计算分子量 584 aa, 62 kDa
观测分子量68 kDa
GenBank蛋白编号BC093972
基因名称 EPN2
Gene ID (NCBI) 22905
RRIDAB_3669948
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95208
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.

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