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验证数据展示

  • WB analysis using 85139-9-RR (same clone as 85139-9-PBS)

    WB analysis using 85139-9-RR (same clone as 85139-9-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85139-9-RR (EPHB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85139-9-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 85139-9-RR (same clone as 85139-9-PBS)

    IF Staining of HT-29 using 85139-9-RR (same clone as 85139-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using EPHB4 antibody (85139-9-RR, Clone: 252186F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85139-9-PBS in a different storage buffer formulation.

  • Affinity of 85139-9-RR

    Affinity of 85139-9-RR

    Biolayer interferometry (BLl) kinetic assays of 85139-9-RR against Human EPHB4 were performed. The affinity constant is 0.295 nM.

EPHB4 Recombinant monoclonal antibody, PBS Only

EPHB4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85139-9-PBS

产品说明书

CloneNo. 252186F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EPHB4 Ready-To-Use IHC Kit. EPHB4 primary antibody included.

产品信息

85139-9-PBS targets EPHB4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6504

Product name: Recombinant Human EPHB4 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 16-539 aa of NM_004444.4

Sequence: LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EPH receptor B4
别名 EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK
计算分子量108 kDa
观测分子量 110-120 kDa
GenBank蛋白编号NM_004444.4
基因名称 EPHB4
Gene ID (NCBI) 2050
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54760
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker.

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