- Featured Product
- KD/KO Validated
EPHB4 Polyclonal antibody
EPHB4 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HT-29 cells |
| Positive FC detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20883-1-AP targets EPHB4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | EPHB4 fusion protein Ag10033 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | EPH receptor B4 |
| Synonyms | EPH receptor B4, EPHB4, Ephrin type B receptor 4, HTK, MYK1, TYRO11, Tyrosine protein kinase TYRO11 |
| Calculated Molecular Weight | 987 aa, 108 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC052804 |
| Gene Symbol | EPHB4 |
| Gene ID (NCBI) | 2050 |
| RRID | AB_10897158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54760 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHB4 antibody 20883-1-AP | Download protocol |
| IHC protocol for EPHB4 antibody 20883-1-AP | Download protocol |
| IF protocol for EPHB4 antibody 20883-1-AP | Download protocol |
| FC protocol for EPHB4 antibody 20883-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Small Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres. | ||
Drug Deliv Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging. | ||
J Cell Physiol Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling. | ||
J Cell Mol Med Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma. | ||
Phytomedicine Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer. | ||
