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EPHB4 Polyclonal antibody

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20883-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

MYK1, HTK, Ephrin type-B receptor 4, Ephrin type B receptor 4, EPH receptor B4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy EPHB4 Ready-To-Use IHC Kit. EPHB4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20883-1-AP targets EPHB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 EPHB4 fusion protein Ag10033 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor B4
别名 MYK1, HTK, Ephrin type-B receptor 4, Ephrin type B receptor 4, EPH receptor B4
计算分子量 987 aa, 108 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC052804
基因名称 EPHB4
Gene ID (NCBI) 2050
RRIDAB_10897158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54760
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPHB4 antibody 20883-1-APDownload protocol
IHC protocol for EPHB4 antibody 20883-1-APDownload protocol
IF protocol for EPHB4 antibody 20883-1-APDownload protocol
FC protocol for EPHB4 antibody 20883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Small

Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.

Authors - Xiaomeng Guo
humanIF

Drug Deliv

Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.

Authors - Zuhua Wang
WB,IHC

J Cell Physiol

Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.

Authors - Tiancheng Li
humanWB

J Cell Mol Med

Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.

Authors - Immacolata Andolfo
humanFC

Phytomedicine

Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.

Authors - Qi Su
ratIHC

Commun Biol

The application of tissue-engineered fish swim bladder vascular graft.

Authors - Hualong Bai
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