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验证数据展示

  • IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 21382-1-AP (same clone as 21382-1-PBS)

    IF Staining of Jurkat using 21382-1-AP (same clone as 21382-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EPHB3 antibody (21382-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

EPHB3 Polyclonal antibody, PBS Only

EPHB3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 21382-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

EK2, EPH like kinase 2, EPH receptor B3, Ephrin type B receptor 3, ETK2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21382-1-PBS targets EPHB3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16016

Product name: Recombinant human EPHB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 261-390 aa of BC052968

Sequence: EWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor B3
别名 EK2, EPH like kinase 2, EPH receptor B3, Ephrin type B receptor 3, ETK2
计算分子量 998 aa, 110 kDa
GenBank蛋白编号BC052968
基因名称 EPHB3
Gene ID (NCBI) 2049
RRIDAB_3669366
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54753
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHB3 is a member of the Eph receptor tyrosine kinase family. The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). The EPHB3 protein has multiple functions in the nervous system, including regulating neural networks, participating in epileptic activity, and playing a role in repair after spinal cord injury.

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