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EPHA8 Polyclonal antibody

EPHA8 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13724-1-AP

Print datasheet

Synonyms

EEK, EK3, EPH and ELK related kinase, EPH like kinase 3, EPH receptor A8, EPHA8, Ephrin type A receptor 8, HEK3, KIAA1459

验证数据展示

  • WB analysis of mouse brain using 13724-1-AP

    WB analysis of mouse brain using 13724-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13724-1-AP (EPHA8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 13724-1-AP

    FC experiment of SH-SY5Y using 13724-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug EPHA8 antibody (13724-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13724-1-AP targets EPHA8 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EPHA8 fusion protein Ag4503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EPH receptor A8
Synonyms EEK, EK3, EPH and ELK related kinase, EPH like kinase 3, EPH receptor A8, EPHA8, Ephrin type A receptor 8, HEK3, KIAA1459
Calculated Molecular Weight 1005 aa, 111 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC038796
Gene Symbol EPHA8
Gene ID (NCBI) 2046
RRIDAB_10638906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPHA8 antibody 13724-1-APDownload protocol
FC protocol for EPHA8 antibody 13724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Oncol Rep

EphA8 inhibits cell apoptosis via AKT signaling and is associated with poor prognosis in breast cancer.

Authors - Gui-Hua Wang
View Article