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验证数据展示

  • WB analysis of human placenta using 84921-3-RR

    WB analysis of human placenta using 84921-3-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84921-3-RR

    IHC staining of human placenta using 84921-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84921-3-RR

    IHC staining of human placenta using 84921-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEL using 84921-3-RR

    IF Staining of HEL using 84921-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EPB42 Recombinant antibody

EPB42 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84921-3-RR

产品说明书

CloneNo. 242412F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEL cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84921-3-RR targets EPB42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 EPB42 fusion protein Ag23556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 erythrocyte membrane protein band 4.2
别名 242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA
观测分子量77 kDa
GenBank蛋白编号BC096093
基因名称 EPB42
Gene ID (NCBI) 2038
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

实验方案

Product Specific Protocols
WB protocol for EPB42 antibody 84921-3-RRDownload protocol
IHC protocol for EPB42 antibody 84921-3-RRDownload protocol
IF protocol for EPB42 antibody 84921-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols