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验证数据展示

  • WB analysis using 10719-1-AP

    WB analysis using 10719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10719-1-AP (EPB41L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included
  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included
  • IHC staining of mouse kidney using 10719-1-AP

    IHC staining of mouse kidney using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10719-1-AP (EPB41L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included

EPB41L3 Polyclonal antibody

EPB41L3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10719-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

4.1B, Band 4.1-like protein 3, Band 4.1-like protein 3, N-terminally processed, DAL1, DAL-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells
Positive IHC detected inhuman brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10719-1-AP targets EPB41L3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1089

Product name: Recombinant human EPB41L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 191-312 aa of BC008377

Sequence: MQCKVILLDGSEYTCDVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDAENQKNWLDPAKEIKKQVRSGAWHFSFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythrocyte membrane protein band 4.1-like 3
别名 4.1B, Band 4.1-like protein 3, Band 4.1-like protein 3, N-terminally processed, DAL1, DAL-1
计算分子量 121 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC008377
基因名称 EPB41L3
Gene ID (NCBI) 23136
RRIDAB_2098488
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2J2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

实验方案

Product Specific Protocols
WB protocol for EPB41L3 antibody 10719-1-APDownload protocol
IHC protocol for EPB41L3 antibody 10719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis

Authors - Min Kyung Kim
View Article