验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Jurkat cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy EPB41 Ready-To-Use IHC Kit. EPB41 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13014-1-AP targets EPB41 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4075 Product name: Recombinant human EPB41 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC039079 Sequence: MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNMHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) |
| 别名 | Erythrocyte membrane protein band 4.1, EPB4.1, EPB 41, Band 4.1, 4.1R |
| 计算分子量 | 720 aa, 81 kDa |
| 观测分子量 | 80 kDa, 135 kDa |
| GenBank蛋白编号 | BC039079 |
| 基因名称 | EPB41 |
| Gene ID (NCBI) | 2035 |
| RRID | AB_2098362 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11171 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for EPB41 antibody 13014-1-AP | Download protocol |
| IP protocol for EPB41 antibody 13014-1-AP | Download protocol |
| WB protocol for EPB41 antibody 13014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cortical Dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.
| ||
EMBO J NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. | ||
Front Oncol Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma. | ||
Int Immunopharmacol Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice | ||
Immunology Cytoskeleton Protein 4.1R Regulates B cell fate by Modulating the Canonical NF-κB Pathway.
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