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验证数据展示

  • WB analysis of CHO using 12572-1-AP (same clone as 12572-1-PBS)

    WB analysis of CHO using 12572-1-AP (same clone as 12572-1-PBS)

    CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

    IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

  • WB analysis using 12572-1-AP (same clone as 12572-1-PBS)

    WB analysis using 12572-1-AP (same clone as 12572-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

EPAC1 Polyclonal antibody, PBS Only

EPAC1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 12572-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12572-1-PBS targets EPAC1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, hamster
免疫原

CatNo: Ag3240

Product name: Recombinant human RAPGEF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 441-788 aa of BC017728

Sequence: HFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rap guanine nucleotide exchange factor (GEF) 3
别名
计算分子量 881 aa, 99 kDa
观测分子量 99-104 kDa
GenBank蛋白编号BC017728
基因名称 EPAC1/RAPGEF3
Gene ID (NCBI) 10411
RRIDAB_11232407
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95398
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

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