验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, Jurkat cells, Raji cells |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13021-1-AP targets ENTPD3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag4114 Product name: Recombinant human ENTPD3 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 45-366 aa of BC029869 Sequence: IHKQEVLPPGLKYGIVLDAGSSRTTVYVYQWPAEKENNTGVVSQTFKCSVKGSGISSYGNNPQDVPRAFEECMQKVKGQVPSHLHGSTPIHLGATAGMRLLRLQNETAANEVLESIQSYFKSQPFDFRGAQIISGQEEGVYGWITANYLMGNFLEKNLWHMWVHPHGVETTGALDLGGASTQISFVAGEKMDLNTSDIMQVSLYGYVYTLYTHSFQCYGRNEAEKKFLAMLLQNSPTKNHLTNPCYPRDYSISFTMGHVFDSLCTVDQRPESYNPNDVITFEGTGDPSLCKEKVASIFDFKACHDQETCSFDGVYQPKIKGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ectonucleoside triphosphate diphosphohydrolase 3 |
| 别名 | Ecto-ATPase 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, EC:3.6.1.5, CD39L3 |
| 计算分子量 | 529 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC029869 |
| 基因名称 | ENTPD3 |
| Gene ID (NCBI) | 956 |
| RRID | AB_2100082 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75355 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ENTPD3 antibody 13021-1-AP | Download protocol |
| WB protocol for ENTPD3 antibody 13021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice. | ||
Reprod Sci Expression and Hormonal Regulation of Entpd3 at Various Estrous Cycle Stages in the Mouse Uterus |
