验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
13656-1-AP targets ENTPD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag4513 Product name: Recombinant human ENTPD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 38-323 aa of BC035738 Sequence: LKYGIVLDAGSSHTSMFIYKWPADKENDTGIVGQHSSCDVPGGGISSYADNPSGASQSLVGCLEQALQDVPKERHAGTPLYLGATAGMRLLNLTNPEASTSVLMAVTHTLTQYPFDFRGARILSGQEEGVFGWVTANYLLENFIKYGWVGRWFRPRKGTLGAMDLGGASTQITFETTSPAEDRASEVQLHLYGQHYRVYTHSFLCYGRDQVLQRLLASALQTHGFHPCWPRGFSTQVLLGDVYQSPCTMAQRPQNFNSSARVSLSGSSDPHLCRDLVSGLFSFSSC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ectonucleoside triphosphate diphosphohydrolase 2 |
| 别名 | CD39 antigen-like 1, CD39L1, EC:3.6.1.-, Ecto-ATP diphosphohydrolase 2, Ecto-ATPase 2 |
| 计算分子量 | 495 aa, 54 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC035738 |
| 基因名称 | ENTPD2 |
| Gene ID (NCBI) | 954 |
| RRID | AB_2877966 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5L3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENTPD2(Ectonucleoside triphosphate diphosphohydrolase 2) is also named as CD39L1, Ecto-ATPase 2 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. This protein has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ENTPD2 antibody 13656-1-AP | Download protocol |
| IP protocol for ENTPD2 antibody 13656-1-AP | Download protocol |
| WB protocol for ENTPD2 antibody 13656-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
