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验证数据展示

  • WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 29862-1-AP (same clone as 29862-1-PBS)

    WB analysis of HeLa using 29862-1-AP (same clone as 29862-1-PBS)

    WB result of ENT1 antibody (29862-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENT1 transfected HeLa cells. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    WB analysis using 29862-1-AP (same clone as 29862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29862-1-AP (ENT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29862-1-AP (same clone as 29862-1-PBS)

    IHC staining of human colon cancer using 29862-1-AP (same clone as 29862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 29862-1-AP (same clone as 29862-1-PBS)

    IHC staining of human lymphoma using 29862-1-AP (same clone as 29862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 29862-1-AP (ENT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29862-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ENT1 Polyclonal antibody, PBS Only

ENT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29862-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ENT-1, Equilibrative NBMPR-sensitive nucleoside transporter, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, es nucleoside transporter, Nucleoside transporter, es-type

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29862-1-PBS targets ENT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32003

Product name: Recombinant human ENT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-82 aa of NM_001372327.1

Sequence: NFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 29 (nucleoside transporters), member 1
别名 ENT-1, Equilibrative NBMPR-sensitive nucleoside transporter, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, es nucleoside transporter, Nucleoside transporter, es-type
计算分子量50KD
观测分子量70 kDa
GenBank蛋白编号NM_001372327.1
基因名称 ENT1
Gene ID (NCBI) 2030
RRIDAB_2935484
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99808
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENT1 (equilibrative nucleoside transporter 1; also known as SLC29A1) is a ubiquitous protein located at the cell membrane and is a major transporter responsible for the cellular uptake and release of endogenous nucleosides such as adenosine, and nucleoside analogs used in chemotherapy. The predicted molecular weight of ENT1 is 50 kDa, while heavier 54-68 kDa band may be observed due to the glycosylation (PMID: 16448802, 11850433, 16111480). A smaller novel splice variant of the mouse ENT1 has also been identified, which generates a protein of 35-40 kDa (PMID: 18413666).

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