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验证数据展示

  • WB analysis of mouse brain using 14518-1-AP

    WB analysis of mouse brain using 14518-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 14518-1-AP

    WB analysis using 14518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 14518-1-AP

    IHC staining of human small intestine using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 14518-1-AP

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 14518-1-AP

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14518-1-AP

    IF Staining of HeLa using 14518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENSA antibody (14518-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ENSA Polyclonal antibody

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14518-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ARPP-19e, Alpha-endosulfine

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HeLa cells
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14518-1-AP targets ENSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ENSA fusion protein Ag5982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endosulfine alpha
别名 ARPP-19e, Alpha-endosulfine
计算分子量 14 kDa
观测分子量 19 kDa
GenBank蛋白编号BC004461
基因名称 ENSA
Gene ID (NCBI) 2029
RRIDAB_10838808
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.

实验方案

Product Specific Protocols
WB protocol for ENSA antibody 14518-1-APDownload protocol
IHC protocol for ENSA antibody 14518-1-APDownload protocol
IF protocol for ENSA antibody 14518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols