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验证数据展示

  • WB analysis using 32226-1-AP

    WB analysis using 32226-1-AP

    Mouse testis tissue lysate was subjected to SDS PAGE followed by western blot with 32226-1-AP (ENPP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ENPP7 Polyclonal antibody

ENPP7 Polyclonal Antibody for WB, ELISA
Cat No. 32226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Alkaline sphingomyelin phosphodiesterase, Alk-SMase, EC:3.1.4.12, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32226-1-AP targets ENPP7 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36493

Product name: Recombinant human ENPP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 150-400 aa of NM_178543.4

Sequence: QGVAVTRSRKEGIAHNYKNETEWRANIDTVMAWFTEEDLDLVTLYFGEPDSTGHRYGPESPERREMVRQVDRTVGYLRESIARNHLTDRLNLIITSDHGMTTVDKRAGDLVEFHKFPNFTFRDIEFELLDYGPNGMLLPKEGRLEKVYDALKDAHPKLHVYKKEAFPEAFHYANNPRVTPLLMYSDLGYVIHGRINVQFNNGEHGFDNKDMDMKTIFRAVGPSFRAGLEVEPFESVHVYELMCRLLGIVPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleotide pyrophosphatase/phosphodiesterase 7
别名 Alkaline sphingomyelin phosphodiesterase, Alk-SMase, EC:3.1.4.12, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7
计算分子量51 kDa，458aa
观测分子量51 kDa
GenBank蛋白编号NM_178543.4
基因名称 ENPP7
Gene ID (NCBI) 339221
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6UWV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (ENPP7) stands out as an alkaline sphingomyelinase that functions in the intestine to cleave sphingomyelin (SM) into ceramide and phosphocholine in a phospholipase C (PLC) -like manner and has been associated with hepatobiliary diseases. Reduced ENPP7 activity is detected in colorectal cancer and in familial adenomatous polyposis, suggesting a link between decreased SM metabolism and colon tumorigenesis (PMID: 22177013; 28292932).

实验方案

Product Specific Protocols
WB protocol for ENPP7 antibody 32226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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