• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ENPEP Polyclonal antibody

ENPEP Polyclonal Antibody for FC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 17655-1-AP

Print datasheet

Synonyms

Aminopeptidase A, AP A, APA, CD249, Differentiation antigen gp160, EAP, ENPEP, Glutamyl aminopeptidase, gp160

验证数据展示

  • IF Staining of HepG2 using 17655-1-AP

    IF Staining of HepG2 using 17655-1-AP

    Immunofluorescent analysis of HepG2 cells, using ENPEP antibody 17655-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • FC experiment of HepG2 using 17655-1-AP

    FC experiment of HepG2 using 17655-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug ENPEP antibody (17655-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17655-1-AP targets ENPEP in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, ELISA
Cited ApplicationsIF, FC
Tested Reactivity human
Cited Reactivitymouse
Immunogen ENPEP fusion protein Ag11903 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamyl aminopeptidase (aminopeptidase A)
Synonyms Aminopeptidase A, AP A, APA, CD249, Differentiation antigen gp160, EAP, ENPEP, Glutamyl aminopeptidase, gp160
Calculated Molecular Weight 957 aa, 109 kDa
GenBank Accession NumberBC094770
Gene Symbol ENPEP
Gene ID (NCBI) 2028
RRIDAB_2099487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ENPEP antibody 17655-1-APDownload protocol
FC protocol for ENPEP antibody 17655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
FC

Small

Engineering of the fluorescent-energy-conversion arm of phi29 DNA packaging motor for single-molecule studies.

Authors - Tae Jin Lee
View Article
mouseIF

Cell Rep

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Authors - Taha A Jan
View Article