验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
55234-1-AP targets ENO3-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, bovine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | enolase 3 (beta, muscle) |
| 别名 | ENO3, MSE, GSD13, EC:4.2.1.11, Beta-enolase |
| 计算分子量 | 47 kDa |
| 观测分子量 | 42-47 kDa |
| GenBank蛋白编号 | NM_001976 |
| 基因名称 | ENO3 |
| Gene ID (NCBI) | 2027 |
| RRID | AB_10837363 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13929 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
| IHC protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
| IF protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteomics Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation. | ||
Ann Transl Med ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.
| ||
Med Oncol ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.
| ||
Reprod Domest Anim Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation. |
