Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28117-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IHC, ELISA and More (1)

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHUVEC cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Endoglin/CD105 Ready-To-Use IHC Kit. Endoglin/CD105 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28117-1-AP targets Endoglin/CD105 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 Human
文献引用反应性human
免疫原 Endoglin/CD105 fusion protein Ag27822 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endoglin
别名 CD105, END, Endoglin, ENG, HHT1, ORW, ORW1
计算分子量 71 kDa
观测分子量 95 kDa
GenBank蛋白编号BC014271
基因名称 CD105
Gene ID (NCBI) 2022
RRIDAB_2881066
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 28117-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 28117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Transl Oncol

A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response

Authors - Xinyu Zhang
humanIHC

Mol Cancer Res

A TGFβ-dependent stromal subset underlies immune checkpoint inhibitor efficacy in DNA mismatch repair-deficient/microsatellite instability-high colorectal cancer.

Authors - Eisei Endo
humanIHC

Sci Rep

Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells

Authors - Kazuma Yoshida
humanIF

Biochem Pharmacol

H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis

Authors - Yupeng Zhao
Human

Mol Cancer Res

Neoadjuvant Chemotherapy Induces IL34 Signaling and Promotes Chemoresistance via Tumor-Associated Macrophage Polarization in Esophageal Squamous Cell Carcinoma

Authors - Shotaro Nakajima
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