验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat kidney tissue, rat liver tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22148-1-AP targets ENDOG in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag17739 Product name: Recombinant human ENDOG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 63-297 aa of BC016351 Sequence: ELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endonuclease G |
| 别名 | Endonuclease G, Endo G, EC:3.1.30.- |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC016351 |
| 基因名称 | ENDOG |
| Gene ID (NCBI) | 2021 |
| RRID | AB_11232230 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14249 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ENDOG antibody 22148-1-AP | Download protocol |
| IP protocol for ENDOG antibody 22148-1-AP | Download protocol |
| WB protocol for ENDOG antibody 22148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Chem Biol Interact O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway. | ||
Inflammation Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro | ||
Front Pharmacol Quercitrin Attenuates Acetaminophen-Induced Acute Liver Injury by Maintaining Mitochondrial Complex I Activity. | ||
Front Pharmacol Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. | ||
Metallomics Induction of mitochondrial apoptosis pathway mediated through caspase-8 and c-Jun N-terminal kinase by cadmium-activated Fas in rat cortical neurons. | ||
Int J Mol Sci Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells. |
