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ENAH Polyclonal antibody

ENAH Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26421-1-AP

Print datasheet

Synonyms

ENA, enabled homolog (Drosophila), ENAH, MENA, NDPP1, Protein enabled homolog

验证数据展示

  • WB analysis of U-251 using 26421-1-AP

    WB analysis of U-251 using 26421-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 26421-1-AP

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 26421-1-AP

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 26421-1-AP

    IF Staining of MCF-7 using 26421-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26421-1-AP (ENAH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 26421-1-AP

    WB analysis using 26421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, U-251 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26421-1-AP targets ENAH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen ENAH fusion protein Ag24200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enabled homolog (Drosophila)
Synonyms ENA, enabled homolog (Drosophila), ENAH, MENA, NDPP1, Protein enabled homolog
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight 85-88 kDa, 75-80 kDa
GenBank Accession NumberBC095481
Gene Symbol ENAH
Gene ID (NCBI) 55740
RRIDAB_2880509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N8S7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ENAH antibody 26421-1-APDownload protocol
IHC protocol for ENAH antibody 26421-1-APDownload protocol
IF protocol for ENAH antibody 26421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols