验证数据展示
经过测试的应用
| Positive WB detected in | unboiled RAW 264.7 cells, HL-60 cells |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse peritoneal macrophages |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27044-1-AP targets EMR1 in WB, IHC, IF/ICC, ELISA, Immunohistochemistry applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Immunohistochemistry |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag25883 Product name: Recombinant human EMR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-510 aa of BC059395 Sequence: TEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | egf-like module containing, mucin-like, hormone receptor-like 1 |
| 别名 | ADGRE1, Adhesion G protein-coupled receptor E1, EGF like module receptor 1, EMR1, EMR1 hormone receptor, F4/80, TM7LN3 |
| 计算分子量 | 886 aa, 97 kDa |
| 观测分子量 | 160 kDa |
| GenBank蛋白编号 | BC059395 |
| 基因名称 | EMR1 |
| Gene ID (NCBI) | 2015 |
| RRID | AB_2716814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14246 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EMR1 (EGF-like module containing mucin-like hormone receptor 1), also known as Adhesion G protein-coupled receptor E1 (ADGRE1), is a surface receptor with seven transmembrane segments that belong to the EGF-7-transmembrane family of G protein-coupled receptors (PMID: 14647991, 7601460). EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans and may be used as a novel therapeutic target for eosinophilic disorders (PMID: 17823986, 24530099). F4/80, the murine homolog of EMR1, is a marker of murine macrophage. The apparent molecular weight of F4/80 is 160 kDa, which is larger than the calculated molecular weight due to post-translational modifications (PMID: 7308288; 8647179).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for EMR1 antibody 27044-1-AP | Download protocol |
| IHC protocol for EMR1 antibody 27044-1-AP | Download protocol |
| WB protocol for EMR1 antibody 27044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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