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验证数据展示

  • WB analysis using 12765-1-AP (same clone as 12765-1-PBS)

    WB analysis using 12765-1-AP (same clone as 12765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12765-1-AP (same clone as 12765-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 12765-1-AP (same clone as 12765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 12765-1-AP (same clone as 12765-1-PBS)

    IHC staining of human stomach using 12765-1-AP (same clone as 12765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

EML1 Polyclonal antibody, PBS Only

EML1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12765-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

HuEMAP-1, EMAP-1, EMAP, Echinoderm microtubule-associated protein-like 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12765-1-PBS targets EML1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3429

Product name: Recombinant human EML1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033043

Sequence: MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTNLCAVDDSND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 echinoderm microtubule associated protein like 1
别名 HuEMAP-1, EMAP-1, EMAP, Echinoderm microtubule-associated protein-like 1
计算分子量 815 aa, 90 kDa
观测分子量 90-92 kDa
GenBank蛋白编号BC033043
基因名称 EML1
Gene ID (NCBI) 2009
RRIDAB_10644132
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00423
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EML1 is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped.

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