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验证数据展示

  • IP experiment of COLO 320 using 21308-1-AP

    IP experiment of COLO 320 using 21308-1-AP

    IP result of anti-EMILIN3 (IP:21308-1-AP, 4ug; Detection:21308-1-AP 1:600) with COLO 320 cells lysate 1920 ug.

  • IHC staining of mouse testis using 21308-1-AP

    IHC staining of mouse testis using 21308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 21308-1-AP

    IHC staining of mouse testis using 21308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EMILIN3 Polyclonal antibody

EMILIN3 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 21308-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IP, ELISA

别名

C20orf130, EMILIN 3, EMILIN 5, EMILIN5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inCOLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21308-1-AP targets EMILIN3 in IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15925

Product name: Recombinant human EMILIN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-766 aa of BC101045

Sequence: DELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elastin microfibril interfacer 3
别名 C20orf130, EMILIN 3, EMILIN 5, EMILIN5
计算分子量 766 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC101045
基因名称 EMILIN3
Gene ID (NCBI) 90187
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NT22
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMILIN3 (Elastin Microfibril Interfacer 3) is a protein-coding gene that encodes an extracellular matrix glycoprotein. It is a member of the EMILIN family and plays a role in the organization of the extracellular matrix, particularly in elastic fiber formation and extracellular matrix organization.

实验方案

Product Specific Protocols
IHC protocol for EMILIN3 antibody 21308-1-APDownload protocol
IP protocol for EMILIN3 antibody 21308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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