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验证数据展示

  • IP experiment of COLO 320 using 21308-1-AP

    IP experiment of COLO 320 using 21308-1-AP

    IP result of anti-EMILIN3 (IP:21308-1-AP, 4ug; Detection:21308-1-AP 1:600) with COLO 320 cells lysate 1920 ug.

  • IHC staining of mouse testis using 21308-1-AP

    IHC staining of mouse testis using 21308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 21308-1-AP

    IHC staining of mouse testis using 21308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21308-1-AP (EMILIN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EMILIN3 Polyclonal antibody

EMILIN3 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 21308-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IP, ELISA

别名

C20orf130, EMILIN 3, EMILIN 5, EMILIN5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inCOLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21308-1-AP targets EMILIN3 in IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15925

Product name: Recombinant human EMILIN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-766 aa of BC101045

Sequence: DELTRLSAAMLEGGVDGLLEGLETLNGTEGGARGCCLRLDMGGWGVGGFGTMLEERVQSLEERLATLAGELSHDSASPGRSARPLVQTELAVLEQRLVSLETSCTPSTTSAILDSLVAEVKAWQSRSEALLRQVASHAALLQQLNGTVAEVQGQLAEGTGSSLQGEITLLKVNLNSVSKSLTGLSDSVSQYSDAFLAANTSLDERERKVEAEVQAIQEQVSSQGSRLQAGHRQVLNLRGELEQLKAGVAKVASGLSRCQDTAQKLQHTVGHFDQRVAQVEGACRRLGLLAAGLDSLPTEPLRPREGLWSHVDQLNRTLAQHTQDIARLRDDLLDCQAQLAEQVRPGQAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elastin microfibril interfacer 3
别名 C20orf130, EMILIN 3, EMILIN 5, EMILIN5
计算分子量 766 aa, 83 kDa
观测分子量83 kDa
GenBank蛋白编号BC101045
基因名称 EMILIN3
Gene ID (NCBI) 90187
RRIDAB_3742026
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NT22
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMILIN3 (Elastin Microfibril Interfacer 3) is a protein-coding gene that encodes an extracellular matrix glycoprotein. It is a member of the EMILIN family and plays a role in the organization of the extracellular matrix, particularly in elastic fiber formation and extracellular matrix organization.

实验方案

Product Specific Protocols
IHC protocol for EMILIN3 antibody 21308-1-APDownload protocol
IP protocol for EMILIN3 antibody 21308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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