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验证数据展示

  • WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

    WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

EMILIN1 Monoclonal antibody, PBS Only

EMILIN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60047-1-PBS

产品说明书

CloneNo. 2A5C8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EMILIN1 Ready-To-Use IHC Kit. EMILIN1 primary antibody included.

产品信息

60047-1-PBS targets EMILIN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 EMILIN1 fusion protein Ag1016 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 elastin microfibril interfacer 1
别名 EMI, EMILIN, EMILIN 1, EMILIN1, gp115
计算分子量 107 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC007530
基因名称 EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2100191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6C2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).