|Positive WB detected in||HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, A431 cells|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human thyroid cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10351-1-AP targets Emerin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Applications||FC, IF, IHC, IP, WB, ELISA|
|Cited Applications||CoIP, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Immunogen||Emerin fusion protein Ag0236|
|Host / Isotype||Rabbit / IgG|
|Synonyms||EDMD, EMD, Emerin, LEMD5, STA|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC000738|
|Gene ID (NCBI)||2010|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
Cytoplasmic control of intranuclear polarity by human cytomegalovirus.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.
Off-the-shelf proximity biotinylation for interaction proteomics.
Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.
J Cell Biol
Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.