验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, mouse heart tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34333-1-AP targets EMCN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Eg6353 Product name: Recombinant mouse Endomucin protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-190 aa of NM_001163522.1 Sequence: EDGKDVQNDSIPTPAETSTTKASVTIPGIVSVTNPNKPADGTPPEGTTKSDVSQTSLVTTINSLTTPKHEVGTTTEGPLRNESSTMKITVPNTPTSNANSTLPGSQNKTENQSSIRTTEISVTTQLLDALPKITATSSASLTTAHTMSLLQDTEDRKIATTPSTTPSYSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endomucin |
| 别名 | Endomucin, Endomucin-1/2, Muc14, MUC-14, Mucin-14 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 70-90 kDa |
| GenBank蛋白编号 | NM_001163522.1 |
| 基因名称 | Emcn |
| Gene ID (NCBI) | 59308 |
| RRID | AB_3743192 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9R0H2-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endomucin (EMCN), also known as Endomucin 2 or Mucin 14, is a type I integral membrane glycoprotein that is endothelial-specific. EMCN is expressed on the surface of capillaries and venous and is highly expressed in highly vascularized tissues such as heart, kidney and lung (PMID: 29215001). Endomucin plays an important role in cell interaction, molecular cell signaling, angiogenesis and cell migration (PMID: 33532708). Since it is glycosylated, the apparent molecular weight of EMCN could be variable, ranging from 70 kDa to 120 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for EMCN antibody 34333-1-AP | Download protocol |
| IHC protocol for EMCN antibody 34333-1-AP | Download protocol |
| WB protocol for EMCN antibody 34333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
